Zagazig University Digital Repository
Home
Thesis & Publications
All Contents
Publications
Thesis
Graduation Projects
Research Area
Research Area Reports
Search by Research Area
Universities Thesis
ACADEMIC Links
ACADEMIC RESEARCH
Zagazig University Authors
Africa Research Statistics
Google Scholar
Research Gate
Researcher ID
CrossRef
Characterization of φEf-vB1 prophage infecting oral Enterococcus faecalis and enhancing bacterial biofilm formation
Faculty
Science
Year:
2020
Type of Publication:
ZU Hosted
Pages:
Authors:
Gamal EL Daydamony Mohamed Bioumy
Staff Zu Site
Abstract In Staff Site
Journal:
Journal of Medical Microbiology Microbiology Society
Volume:
Keywords :
Characterization , φEf-vB1 prophage infecting oral Enterococcus
Abstract:
Introduction. Enterococcus faecalis is a facultative, anaerobic, opportunistic pathogen associated with medical and dental diseases. Bacterial phenotypic traits and pathogenesis are often influenced by lysogeny. Aim. The aim of this study was to characterize both the morphology and complete genome sequences of induced prophages purified from E. faecalis clinical isolates. Methodology. E. faecalis isolates were recovered from the roots of teeth of patients attending an endodontic clinic. The morphological features of isolated phage were characterized using transmission electron microscopy (TEM). DNA sequencing was performed using the Illumina MiSeq platform. Results. TEM indicated that the isolated φEf-vB1 prophage belongs to the family Siphoviridae. The φEf-vB1 prophage was stable over a wide range of temperatures and pH. Sequencing of φEf-vB1 DNA revealed that the phage genome is 37 561 bp in length with a G+C content of 37.6mol% and contained 53 ORFs. Comparison with previously predicted prophage genomes using blast revealed that φEf-vB1 has a high sequence similarity to previously characterized phage genomes. The lysogenic E. faecalis strain exhibited a higher biofilm formation capacity relative to the non-lysogenic strain. Conclusion. The current findings highlight the role of lysogeny in modification of E. faecalis properties and reveal the potential importance of prophages in E. faecalis biology and pathogenesis.
Author Related Publications
Gamal EL Daydamony Mohamed Bioumy, "New Approach to Use Phage Therapy against Aeromonas hydrophila Induced Motile Aeromonas Septicemia in Nile Tilapia", omicsonline, 2016
More
Gamal EL Daydamony Mohamed Bioumy, "OCCUReNCE OF BACILLUS thuringienseis and their phage in yemen soil", springer, 2013
More
Gamal EL Daydamony Mohamed Bioumy, "Occult hepatitis B infection in Egyptian health care workers", WHO EASTERN MEDITERRANEAN REGIONAL OFFICE, P. O. BOX 7608, NASR CITY, CAIRO, EGYPT, 2017
More
Gamal EL Daydamony Mohamed Bioumy, "Predictors of the Therapeutic Response to Intralesional Bivalent HPV Vaccine in Wart Immunotherapy", MPDI, 2021
More
Gamal EL Daydamony Mohamed Bioumy, "Adjuvant effect of saponin in an oil-based monovalent (serotype O) foot-and-mouth disease virus vaccine on the antibody response in guinea pigs and cattle", Springer Nature, 2021
More
Department Related Publications
Mohamed Farouk Ibrahim Aly, "Production of Cellulases and Xylanase by Thermophilic and Alkaliphilic Bacterial Strains Isolated from Agricultural Wastes", World Applied Sciences Journal, 2013
More
weSam AbelGhani Aly, "Biosorption of Some Heavy Metal Ions Using Bacterial Species Isolated from Agriculture Waste Water Drains in Egypt", INSInet Publication, 2009
More
Fifi Mohamed Reda Mohamed, "Detoxification of enterotoxigenic Bacillus cereus (JX455159) isolated from meat by a local strain of Lactobacillus plantarum (JX282192)", springer, 2013
More
Fifi Mohamed Reda Mohamed, "Purification and characterization of a metallo-protease produced by Exiguobacterium sp. S8 (AB813715) isolated from soil", Egypt, 2013
More
Ashraf Sabry Abdelfattah alsaied, "L-glutaminase production by Trichoderma koningii under solid state fermentation", Springer, Association of Microbiologists of India 2009,, 2008
More
جامعة المنصورة
جامعة الاسكندرية
جامعة القاهرة
جامعة سوهاج
جامعة الفيوم
جامعة بنها
جامعة دمياط
جامعة بورسعيد
جامعة حلوان
جامعة السويس
شراقوة
جامعة المنيا
جامعة دمنهور
جامعة المنوفية
جامعة أسوان
جامعة جنوب الوادى
جامعة قناة السويس
جامعة عين شمس
جامعة أسيوط
جامعة كفر الشيخ
جامعة السادات
جامعة طنطا
جامعة بنى سويف