Babesia bovis, Babesia bigemina, Theileria annulata are the most important endemic blood parasites in Ismailia and Sharkia Provinces, Egypt, which transmitted by ticks causing babesiosis and theileriosis which characterized by fever, enlarged lymph nodes, pale mucous membranes and may lead to death. Therefore, the aim of our study is to determine the infection rates of B. bovis, B. bigemina and T. annulata in relation to age. We found that the rates by staining method were as the following: 12.90%, 6.45%, 38.71% and 4.84% for B. bovis, B. bigemina, T. annulata and mixed infection, respectively, while by polymerase chain reaction (PCR) the rates were 35%, 20%, 60% for B. bovis, B. bigemina and T. annulata, respectively. This study confirmed the incidence of infection with Babesia and Theileria species in relation to age group of animals was 77.78% in adult age group, 66.67% in less than 3 months age group and 53.19% in 3-6 months age group. Also, our study proved that the use of imidocarb and buparvoqune beside acaricides considered a good choice for treatment. Although our results proved that using of specific primers in PCR was a rapid and an accurate method for differential diagnosis between Babesia and Theileria species in cattle, we found that the use of staining method was more helpful especially in mixed infection cases. In the end, we recommend using both methods together to get better results.