Imidacloprid is known to induce soil pollution. Herein, we improved phytoremediation of soil contaminated with imidacoprid using Plantago major plus effective microorganisms (EM1) and peat-moss under laboratory conditions for 14-days and using P. major inoculated with EM1 in tomato field for 10-days. Concentration of imidacloprid in soil, roots and leaves was determined. Concentrations of Chlorophyll in leaves were also determined. Our results showed that lower imidacloprid degradation rate was observed in amended soil with peat–moss, while higher imidacloprid degradation rate was observed in soils vegetated with P. major, P. major inoculated by EM1, and P. major amended with peat–moss, respectively. However, degradation rate was high in the case of interaction between soil planted with P. major plus both of EM1 and peat-moss. Concentration of imidacloprid in P. major roots amended with EM1, peat–moss and both was significantly lower than that of P. major roots alone within 1–14 days of treatment. EM1 plus P. major and peat- moss plus P. major had increase of Chlorophyl content in leaves. Cultivation of P. major next to tomatoes crop and inoculated with EM-1 was found to be the most effective process for removing imidacloprid from the soil.