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Distribution of genes encoding adhesins and biofilm formation capacity among Uropathogenic Escherichia coli isolates in relation to the antimicrobial resistance
Faculty
Pharmacy
Year:
2020
Type of Publication:
ZU Hosted
Pages:
Authors:
Amira Mohamed Mohamed Ali Elganayny
Staff Zu Site
Abstract In Staff Site
Journal:
African Health Sciences Makerere University, Medical School
Volume:
Keywords :
Distribution , genes encoding adhesins , biofilm formation
Abstract:
Background: Escherichia coli is the most predominant pathogen involved in UTIs. Mainly, fimbrial surface appendages are impli-cated in adherence to urothelium besides non-fimbrial proteins. Objectives: To determine prevalence of genes encoding fimbrial and non-fimbrial proteins among Uropathogenic Escherichia coli (UPEC). Furthermore, distribution of these genes and biofilm formation capacity were investigated in relation to antimicrobial resistance. Methods: Antimicrobial susceptibility of 112 UPEC isolates was performed using disc diffusion method. ESBL production was confirmed by double disc synergy test. Genes encoding fimbrial and non-fimbrial proteins were detected using PCR and biofilm formation was investigated using microtitre plate assay. Results: UPEC isolates exhibited high resistance against doxycyclines (88.39%), β-lactams (7.14-86.6%), sulphamethoxaz-ole–trimethoprim (53.75%) and fluoro-quinolones (50%). Fifty percent of tested isolates were ESBL producers. PapGII gene was statistically more prevalent among pyelonephritis isolates. SfaS, focG and picU genes were statistically associated with flu-oro-quinolone (FQs) sensitive isolates and Dr/afaBC gene was statistically associated with ESBL production. Moreover, non-MDR isolates produced sturdier biofilm. Conclusion: PapGII adhesin variant seems to have a critical role in colonization of upper urinary tract. There is a possible link between antimicrobial resistance and virulence being capable of affecting the distribution of some genes besides its negative impact on biofilm formation
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Department Related Publications
Eman Mahmoud Hassan Elmasry, "Studies on Chemoantibiotics Resistant patterns linked to Bacteriocin Production in Clinical Isolates of Gram -Negative Rods .", لايوجد, 1900
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Mona Abdelmoniem Elsayed Mahmmoud , "Effects of some quinolones in adhesiom , haemagglutination , outer membrane proteins and plasmid prophile of uropathogenic Escherichia coli ", لايوجد, 1900
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