There is a needto continue research to find out other anti-dermatophytic agents to inhibit the causal pathogenic skin diseases such as many types of tinea. Production, purification and identification of an anti-dermatophytic substance by Streptomyces atrovirenswere done. Out of 103 streptomycete isolates tested, only 20 of them showed antidermatophytic activity with variable degrees against TrichophytontonsuransCCASU 56400 (T. tonsurans), MicrosporumcanisCCASU 56402 (M. canis)and TrichophytonmentagrophytesCCASU 56404 (T. mentagrophytes). The most potent isolate S10Q6 was identified based on the tests conducted identifying morphological, physiological characteristics and using 16S rRNA gene sequencing. The isolate was found to be closely correlated to previously described species Streptomyces atrovirens; it was designated Streptomyces atrovirensKM192347(S. atrovirens). Maximum antifungal activity of the strain KM192347 was obtained in modified starch nitrate medium (MSNM) adjusted initially at pH 7.0 and incubated at 30 °C in shaken cultures (150 rpm) for 7 days. The antifungal compound was purified by using two steps protocol including solvent extraction and column chromatography. The MIC of it was 20µg/mL against thedermatophyte cultures tested. According to the data obtained from instrumental analysis and surveying the novel antibiotics database, the antidermatophytic substance produced by the strain KM192347 was characterized as an oxaborole-6-benzene sulphonamide derivative and designated OXBS with the chemical formula C13H12 BNO4S. The crude OXBS didn't show any toxicity on livingcells. Finally the results obtained herein described other anti-dermatophytic substance namedan oxaborole-6-benzene sulphonamide derivative (OXBS).