one thousand, 14 day-old Pekin ducklings suffered from depression, eye closing, watery diarrhea and the affected bird fall on their side and kick spasmodically with head stretched upward and backward (opsithotonos position) with mortality rate reached up to 80% reared in kaliobeya governorate were the subject of our investigation. Specimens from different organs including liver spleen, lungs, intestine, heart, gizzard, proventriculus, pancreas, brain and bursa were collected and subjected to the pathology The collected specimen were fixed in 10% neutral buffered formalin solution, dehydrated in gradual alcohol (70-100%), cleared in xylene and embedded in paraffin. Five microns thickness paraffin sections were prepared and stained with hematoxylin and eosin (HE) dyes then examined microscopically. Some of the collected specimens were stored frozen at -20°C to be subjected Reverse - transcriptase polymerase chain reaction (RT-PCR) for the detection of Duck viral hepatitis (DVH). Macroscopically, the liver was enlarged, yellowish brown in color with hemorrhagic spots on its surface, the kidneys, spleen and lungs were enlarged and congested. The brain showed congestion in meninges with hemorrhagic patches beside congestion of intestinal mesentery was observed. Hemorrhage, congestion, cell swelling, necrosis with infiltration of heterophiles and lymphocytes were observed microscopically in the examined organs. PCR technique was positive for Duck viral hepatitis and it was the best tool to diagnose the disease