One thousand, 14-days-old, broiler chickens with respiratory manifestations and a high mortality and high morbidity in Kaliobeya Governorate were the subject of our investigation. Specimens from different organs including trachea, air sacs, lungs, kidneys, liver, heart, brain and spleen were collected and subjected to pathology. The collected specimen were fixed in 10% neutral buffered formalin solution, dehydrated in gradual alcohol (70- 100%), cleared in xylene and embedded in paraffin. Five microns thickness paraffin sections were prepared and stained with hematoxylin and eosin (HE) dyes then examined microscopically. Some of the collected specimens were stored frozen at -20°C to be subjected to real-time polymerase chain reaction (RT-PCR) for the detection of infectious bronchitis virus (IBV). Macroscopically, the trachea showed catarrhal tracheitis represented by congestion and catarrhal exudate inside its lumen with pneumonic area has been observed in lungs. The Kidneys were enlarged, inflamed and the ureters were distended with urates. Necrosis of the epithelial lining of trachea besides hemorrhages, congestion and necrosis with heterophils` and lymphocytes` infiltrations were detected microscopically in kidneys with bronchopneumonia in lungs. Real-time polymerase chain reaction (RT-PCR) was accurate, rapid and sensitive diagnostic tool for detection of IBV in examined chicken organs. The aim of the present study was directed for fully illustration of the histopathological lesions of IBV in broilers as one of the major economically important poultry diseases in Egypt.