Abstract Background: Umbilical cord blood is a readily available source of hematopoietic stem cells that treat a variety of malignant and non-malignant diseases in children and adult. Nurses as frontline health care professionals need to understand umbilical cord stem cells and their application in medicine. Aim of the study: To evaluate the effect of educational intervention on nursing students' performance regarding umbilical cord blood collection for stems cells. Research design: A quasi-experimental research design was used with pre/posttests. Setting: The study was conducted at the faculty of Nursing, Zagazig University. Subjects: 100 undergraduate nursing students at the fourth academic year were recruited for the study. Tools of data collection: Three tools were used. Tool (I): A student nurse self-administered questionnaire which consisted of four parts. Part 1: For collecting data pertaining to students demographic characteristics. Part 2: Includes questions regarding student’s knowledge about umbilical cord. Part 3: Deals with knowledge about haematopoietic stem cells. Part 4: Concerned with knowledge about umbilical cord blood collection. Tool (II): Nurses student's attitudes towards cord blood collection. Tool (III): Observational checklist. Results: It was found that 90% of the students had poor knowledge before intervention which decreased to 0% and 1.8% immediately after and three months later of intervention respectively. Also, only 22% of the studied students had a positive attitude before intervention, which changed to 97% and 89% respectively at immediately after and three months later of intervention. In addition, all students(100%) had poor practices toward the collection of cord blood at pretest which changed to 0% and 3.6% at the post and follow up test with a highly statistically significant improvements (p <0.001) which justify the research hypothesis of intervention. Conclusion: Educational intervention significantly get out improvements in pre, post-tests and after three months for students' knowledge, attitudes and practices regarding umbilical cord blood collection for stem cells. Recommendations: Umbilical cord blood collection and any current technologies must be incorporated into nursing curriculum in order to update the future nurse with this current medical innovation and evidences. Key words: Umbilical cord, blood cord collection, stem cells, nursing students.