Scorpio maurus is one of the most common scorpions encountered in different habitat in Egypt. Recently, many bioactive compounds were isolated from the venom of this species which have the potential for being developed into effective and environmentally friendly bio-insecticides. Anti- Hepatitis C virus and anti-Dengue (DENV) virus agents were also charcacterized from its venom. Moreover, Spermaurin isolated from the venom of this species was found to improve sperm motility and fertilization in various species. Therefore, it is of great importance to describe the structure and function of the venom glands of this important scorpion. The present study was carried out to critically describe the morphology, histology, histochemistry and ultrastructure of venom glands of S. maurus important scorpion. Microscopical examination revealed that the venom apparatus of L. quinquestriatus is composed of paired venom glands situated inside the telson vesicle. Two venom ducts extends from these glands to open at the subterminal sides of the stinger. SEM revealed the presence of many sensory setae over the cuticle of venom apparatus which may have sensory functions. The venom epithelium is simply folded and has three types of cell: venom-producing cells, mucous (goblet) cells and supporting cells. Ultrastructural architecture of these cells and their granules were described. Three types of distinct secretory granules were found in venom producing cells that gave a variable positive response with histological and histochemical stains reflecting their constituent’s richness. The herein results revealed also that these granules are distinct types and no transitional stages reflecting that they are maturation stages were observed. The mode of venom secretion from venom glands of the studied species were found to be of apocrine type