Modulation of the catalytic activity of a metallonuclease by tagging with oligohistidine

Faculty Science Year: 2020
Type of Publication: ZU Hosted Pages: 11
Authors:
Heba Alaa El Din Hosseini Abdel Hamid
Journal: Journal of Inorganic Biochemistry Elsevier Inc Volume: Volume 206
Keywords : Modulation , , catalytic activity , , metallonuclease , tagging with oligohistidine    
Abstract:
Peptide tags are extensively used for affinity purification of proteins. In an optimal case, these tags can be completely removed from the purified protein by a specific protease mediated hydrolysis. However, the interactions of these tags
   
     
 
       

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  • Heba Alaa El Din Hosseini Abdel Hamid, "Purification of proteins with native terminal sequences using a Ni(II)- cleavable C-terminal hexahistidine affinity tag", Protein Expression and Purification, 2019 More

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