The attention to renewable energy increasing as a result of global energy security problems, climate change, and environmental pollution, therefore we found that the rates of renewable energy applications are increasing progressively over the years. In general canola oil can be utilized as an energy source through transesterification which increases its value. This research is aimed to appreciate the feasibility of using canola oil with the wastewater through biological technology. Canola oil should induce high methane production potential but this does not happen without certain drawbacks. For this purpose, canola oil was co-anaerobic digested with wastewater under different loading rates, a continuous stirred tank reactor (CSTR) was used for anaerobic digestion, and the experiment was conducted for 60 days at 37˚C. Hence, evaluate digestion conditions, stability, and affect the overall operational efficiency depending on canola oil characteristics. The results showed that it was noticed that the biogas production varied from 620.12 –1569.20 l/kgots.day depending on OLR 1-3 goTS l -1 d-1.The highest biogas production was 47 l/day with methane content 69.19% when the loading rate was 3 goTS l -1 d-1. Co-digested canola oil with wastewater produce a methane yield that may reach up to 47 l/day with methane content 69.19%, the highest one produced by 3 g oTS l -1 d-1 While increasing loading rate lead to increase VFA, decrease pH level and the production. The ratio of output /input energy reached to1.9 if canola oil used as biogas substrate but be 1.3 when canola oil used as biodiesel source.