Background and Objective: Diagnosis of the internal abscesses is important to relieve the cost burden on animals’ owners that comes from long duration of unspecific and conservative medications and to nourish the animals welfare and hence their productivity. Therefore, this study aimed to evaluate the role of ultrasound, as non-invasive and decision-making tool, in addressing this issue. Materials and Methods: Nineteen buffalo cases were employed in this study and subjected to critical clinical and ultrasonographic examination: pre- and post-therapeutic interventions. Results: Emaciation, inappetance, scanty feces, ruminal atony, systemic disturbances and dull demeanor were found general clinical findings. Specifically, recurrent tympany and abdominal pain (n = 9; 47.4%) and abdominal distension (n = 2; 10.5%) were observed in abdominal abscesses, while respiratory manifestations (n = 6; 31.6%) and cardiac manifestations (n = 4; 21.1%), were recorded in thoracic abscesses. Ultrasonographically, abdominal (n = 13; 68.4%) and thoracic (n = 6; 31.6%) abscesses were diagnosed and the treatment strategies were consequently determined. Of 19 cases, 10 animals (52.6%) were of bad prognosis and advised to undergo slaughter, 6 cases (31.6%) were locally and systemically treated and 3 buffaloes (15.8%) were only subjected to systemic treatment. Of 9 treated animals, 4 cases (44.4%) responded and showed a clear improvement post-treatment. Conclusion: This study concludes that abdominal and thoracic ultrasound is a beneficial tool in diagnosis of buffaloes in which the internal abscessations are obscured. Additionally, it could efficiently provide clinicians with the therapeutic decision for a proper and economical intervention.