A laboratory tests carried out to evaluate nematicidal activities of three fish waters (mature tilapia, fry tilapia and fry mullet) on IJs and egg masses of root-knot nematodes Meloidogyne incognita under laboratory conditions. After two weeks of exposure, fry tilapia water significantly (P ≤ 0.05) more effect one with egg hatching inhibition 24.30% followed by big tilapia water with 19.28% while, fry mullet was less toxic to egg masses of M. incognita. On the other hand, mortality percentages reached to 84.67, 66.00 and 62.67 with fry tilapia fish, big tilapia fish and fry mullet, respectively after two weeks. Two greenhouse experiments were conducted to evaluate the nematicidal activity of fry tilapia fish water alone or combined with composted chicken manure water on galling and reproduction of M. incognita infecting tomato plants (Solanum lycopersicum L. c.v. Super strain B) under greenhouse conditions. Pots irrigated with fry tilapia fish water significantly (P ≤ 0.05) minimized the root-gall numbers (125.67) with percentages of reduction (24.44%) as compared to positive control treatment irrigated with tap water and increased of shoot fresh weight of tomato plants (34.80%). Whereas, galls diameter (≥ 4 mm) decreased to reach 6.00 as compared with 20.00 in pots irrigated with tab water. Pots treated with fry tilapia fish water combined with soaked local chicken manure (local chicken manure water) showed least gall numbers and reproduction of root knot- nematode, M. incognita. Also, statistical analysis showed that fish water + composted local chicken manure water and fish water decreased galls diameter. Since number of galls (≥ 4 mm) decreased to reach 6.00 and 0.00 with fish water and fish water + composted local chicken manure water, respectively as compared with 20.00 in pots irrigated with tab water. Regarding the efficiency of the treated materials on egg masses, results clearly showed that fish water + composted local chicken manure water achieved the highest percentage of reduction in egg masses (68.64%). On the other hand, use fish water alone or fish water + local chicken manure water significantly increase root weight of tomato plants. Percentages of increase in root weight for treated waters were 50.37 and 35.00 % with fish water + local chicken manure water and fish water only, respectively. Also, percentage increase in shoot fresh weight in treatment of fish water + composted local chicken manure water was 77.46% as compared with 34.80% in fish water.