Objective: To evaluate the effect of different pH media on zirconia-reinforced lithium silicate glass ceramic and how they interact with opposing dentition after being aged in different pH cycling and high temperature conditions. Methods: Twenty-five rectangular shaped specimens were prepared from lithium silicate reinforced with zirconia blanks (Suprinity, Vita Zahnfabrick) and stored in different pH media (3 & 7.2) for different periods (24h & 7 days) at temperature (55°C). After their surface roughness (Ra) evaluation, aged ceramic specimens were subjected to cyclic abrasive wear with opposing natural teeth enamel for 150,000 cycles using a chewing simulator. Weight loss and Scanning Electron Microscope (SEM) images were used to evaluate the cyclic wear results. Results: After different pH storage, ceramic group stored at 3 pH for 1-W (1 week) gave significantly higher mean Ra value (0.618μm±0.117) than control lowest mean value (0.357μm±0.054) before cyclic wear. On the other hand, it caused the least significant weight loss value (0.004gm±0.001) to opposing tooth enamel. There was significant tooth enamel weight loss (0.043gm±0.004) when opposed with ceramic group stored in 3 pH media for 24h (24-H). Their SEM images showed a prominent wear scar on enamel cusp tip. There was a significant increase in surface roughness Ra of ceramic material after abrasive cyclic wear. Significance: Great attention should be paid to Ra of this type of glass ceramic even if it is considered as minimal values. It can induce a significant amount of enamel tooth wear after a period equivalent to one year of intra-oral function rather than the significantly higher surface Ra of such ceramic type can do.