Remineralization ability of two hydraulic calcium-silicate based dental pulp capping materials: Cell-independent model

Faculty Faculty of Dentistry Year: 2019
Type of Publication: ZU Hosted Pages:
Authors:
Journal: Juornal of Clinical and Experimental Dentistry Medicina Oral S.L Volume:
Keywords : Remineralization ability , , hydraulic calcium-silicate based dental    
Abstract:
Background: This study aimed to evaluate remineralizing ability of two hydraulic calcium-silicate cements (Bio- dentine and TheraCal LC). Material and Methods: Arti cial carious lesions were introduced into the pulpal oors (1-1.5 mm in thickness) and axial walls of occlusal prepared cavity halves through pH cycling. Cycling was made through demineralizing solution (pH 3), for 8 hours and remineralizing solution (pH 7) for 16 hours. The total period of pH cycling was 14 days. Prepared cavities with the tested materials seated directly on the pulpal oors and in contact with the axial walls were stored in phosphate buffer solutions (PBS) (pH 7.2–7.4). The changes in the weight percentages (wt%) of calcium (Ca) and phosphorus (P) were detected using SEM and energy dispersive X-ray spectroscopy with reference to sound dentin after three intervals (one week, 3 and 6 months). Data were statistically analyzed using two-way ANOVA and Tukey’s post hoc test. Results: Demineralized dentin, next to Biodentine, showed statistically higher intensities of Ca and P wt% after the three periods of incubation (p < 0.05). Surface mapping of both tested cements and their adjacent deminerali- zed dentin showed increase in overall distribution of previous ions. SEM of subsurface layer under both materials showed lling of most intra-tubular areas with rod-like mineralized structure without signi cant difference. Conclusions: Biodentine has a higher ability to enrich the arti cial carious dentin with signi cantly higher mineral contents available for remineralization. Both pulp-capping materials have signi cantly induced remineralization of demineralized dentin beneath them after total period of incubation.
   
     
 
       

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