The toxic properties of remaining dead Anisakis larvae may play important role in human sickness. The aim of this work is to determine the possible impact of exposure to morphologically identified larvae of Anisakis simplex and its antigen on the health condition by measuring of some biochemical, hematological and immunological analytes besides histopathological studies. Sixty rats were divided into equal four groups. Group 1 as a control, group 2 rats were injected intraperitoneal by A. simplex larvae crude antigen (250 μg/rat), group 3 rats were inoculated orally by freshly collected A. simplex larvae (ten larvae /rat) and group 4 rats were inoculated orally by frozen A. simplex larvae at -20°C for 6 months (ten larvae /rat). The animals were injected and inoculated at the zero and seventh day of the experiment. Blood and tissue samples were collected at the 7th and 14th days for performing different tests. Most changes in biochemical and hematological parameters were observed at 14th day of the experiment especially in groups inoculated orally with larvae. Animals injected by larvae crude antigen showed these changes early at 7th day of the experiment. Serum immunoglobulin E levels showed highly significant increase in all experimental groups at 7th day of the experiment. Histopathological changes confirmed the results. These results suggest that the consumption of parasitized fish with A. simplex larvae even in dead condition and after freezing can cause human health disturbance. More sweeping measures than the ones present currently upon consumption of fish must enforce to protect the public.