The current research aimed to identify the effectiveness of using a teaching model based on brain-based learning in developing Higher Order Thinking skills and academic achievement in science among second-grade students preparatory. A research sample was chosen consisting of (64 females) from Om- El Abtal preparatory school for girls, West Zagazig Educational Administration - Sharkia Governorate, first semester 2016-2017. The research sample was divided into two groups: an experimental group (32 females) studied the second and third units in the science book using the teaching model based on brain-based learning, and a control group (32 females) studied the same Units with the usual way. Through that the pre and post application of research tools (Higher Order Thinking Scale- Achievement Test) on two research groups were applied. and several conclusions were reached: Superiority of the experimental group students in the post application on pre application with a statistically significant difference at 0,01 level in the development of Higher Order Thinking and Achievement, and Superiority of the experimental group students on the control group students in the post application with a statistically significant difference at 0,01 level in the development of Higher Order Thinking and Achievement, and some recommendations and proposals were presented in light of the results reached.