Comparison of different techniques and stains for direct diagnosis of Trichinella spiralis

Faculty Veterinary Medicine Year: 2020
Type of Publication: ZU Hosted Pages:
Authors:
Journal: Microbes and Infectious Diseases Zagazig University Volume:
Keywords : Comparison , different techniques , stains , direct diagnosis    
Abstract:
The aim of Trichinella inspection is to reliably detect larvae in meat at levels which are capable of causing human trichinellosis. Trichinoscopy is a rapid, cost effective but is less sensitive in detection of low muscle larvae (ML)numbers. Artificial digestion is more convenient, and flexible, but may destroy immature larvae. Baermann technique may enhance the efficiency of diagnosis. Giemsa and Leishman stains provide identical contrasting coloration as Hematoxylin and Eosin (H-E) with the both advantages of that they can be used for staining non-histological sectionsand rapid preparation.The primary aim of the study was to compare the efficiency of digestion and Baermann techniques for detection of ML in muscles. Secondary aim was detection of ML by Geimsa and Leishman stain and their comparison with routinely used stain H-E. Methods: Muscles from 36 mice orally infected with Trichinella spiralis encysted larvae were examined. Larvae from 1 g samples of different muscles were recovered by digestion and Baermann techniques after different dose of infection. Muscle samples were prepared for staining with Giemsa, Leishman and H-E. Results: Number of larvae recovered by Baermann was higher recovered by digestion method (P ˃0.5). Leishman stain was the best stain for rapid detection of ML after 30 min followed in validity by Geimsa then H-E. Conclusion: Baermann concentration technique showed higher sensitivity than digestion method. Leishman stain is superior to Giemsa stain as it takes less time. Both stains are considered good alternative to H-E for rapid and easy diagnosis of trichinellosis in post-mortem animals.
   
     
 
       

Author Related Publications

  • Mohammed Samer Ahmed Mouhamed, "Virulotyping and genetic diversity of Aeromonas hydrophila isolated from Nile tilapia (Oreochromis niloticus) in aquaculture farms in Egypt", Elsevier, 2021 More
  • Mohammed Samer Ahmed Mouhamed, "Prevalence, protein analysis and possible preventive measures against zoonotic anisakid larvae isolated from marine Atherina fish", Helminthologia, 2015 More
  • Mohammed Samer Ahmed Mouhamed, "MicroRNAs in the Host Response to Viral Infections of Veterinary Importance", Frontiers, 2016 More
  • Mohammed Samer Ahmed Mouhamed, "Small Non-coding RNAs Associated with Viral Infectious Diseases of Veterinary Importance: Potential Clinical Applications", Frontiers, 2016 More
  • Mohammed Samer Ahmed Mouhamed, "Aeromonas hydrophila from marketed mullet (Mugil cephalus) in Egypt: PCR characterization of β-lactam resistance and virulence genes", Wiley, 2018 More

Department Related Publications

  • Mohammed Naser Mohamed Ali, "Survival of Foot and Mouth disease virus strain O, / 2 /72 in Egypt outside the animal body", لايوجد, 1900 More
  • Heba Ahmed Abdallah Abdelmgeed, "A comparative evaluation of PCR- based methods for species- specific determination of African animal trypanosomes in Ugandan cattle", BioMed Central Ltd., 2013 More
  • Maysa Abdelbadea Ibrahiem Awaadallah , "تأثير بعض المطهرات الكيميائية شائعة الاستخدام و الأشعة فوق البنفسجية على خمسة من الملوثات الفطرية ذات الاهمية البيطرية والصحية واسعة الانتشار فى مساكن الماشية", Fac. of Veterinary Med. Zagazig University, Egypt, 2010 More
  • Mohammed Alsayed MohamedMohamed , "Further studies on the role of cray fish (Procambarus clarkia) in transmission of some zoonotic parasites in East Delta دراسات متقدمة عن دور كابوريا المياه العذبة فى نقل بعض الطفيليات المشتركة بشرق الدلتا", كلية الطب البيطرى - جامعة الزقازيق, 2003 More
  • Mohammed Alsayed MohamedMohamed , "Effect of Interferon-α on bovine Rota virus. تأثير انترفيرون الفا على فيروس روتا", كلية الطب البيطرى - جامعة بنها, 2010 More
Tweet