Bovine theileriosis listed one of the tick-borne diseases of crucial concern worldwide.. The objectives of this study were to investigate the occurrence of tropical theileriosis in a farm of cattle demonstrating acute manifestations by Giemsa stained blood smears and Tams1gene based polymerase chain reaction (Tams-1 PCR). Moreover, to follow up the treatment with buparvaquone and long-acting oxytetracycline on the clinical, parasitological and hematological profile of infected cattle. Blood samples were collected from cattle (n=25) suspecting Theileria annulata (T. annulata) infection and healthy ones (n = 10; clinically and parasitologically free) in Sharkia Governorate Egypt, during JulyAugust 2019. Fever (40-41°C), superficial lymph nodes enlargement, corneal opacity, and cases of non-specific abortion were the most manifestations recognized