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New colorimetric methods for microdetermination of melatonin in pure and in dosage forms
Faculty
Science
Year:
1999
Type of Publication:
Article
Pages:
1421-1434
Authors:
ZAKY, M, Amin, AS, Khater, HM, El-Beshbeshy, AM
DOI:
10.1080/00032719908542907
Journal:
ANALYTICAL LETTERS MARCEL DEKKER INC
Volume:
32
Research Area:
Chemistry
ISSN
ISI:000080366200012
Keywords :
melatonin, pharmaceutical formulation, 1, 10-phenanthroline, 2, 2(-)-bipyridyl, colorimetric
Abstract:
Two simple, rapid, sensitive and accurate methods (A and B) for the microdetermination of melatonin in pure form and in pharmaceutical formulations are developed. Method A is based on the formation of tris(o-phenanthroline)iron(II) complex (ferroin) upon reaction of melatonin with an iron(III)-o-phenanthroline mixture in sodium acetate-acetic acid buffer media. The ferroin complex is colorimetrically measured at lambda(max) 510 nn against reagent blank Method B is based on the reduction of Fe (III) by the drug which forms a colored complex (lambda(max) 522 nm) with 2,2(-)-bipyridyl. Optimizations of the experimental conditions are described. Beer's law is obeyed in the concentration range 0.4 - 6.4 mu g ml(-1) for A and 0.4 - 7.4 mu g ml(-1) for B, respectively. The developed methods are applied successfully for the determination of melatonin in pure-form and in melatonin tablets without any interference from common excipients.
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