Heterocyclization of furan-2-carbonyl isothiocyanate 1 with a variety of aliphatic and aromatic nitrogen nucleophiles resulted in the formation of a new series of heterocycles including triazines, pyrimidines, oxadiazines, imidazolidines, thiadiazoles and their condensed candidates. The antibacterial screening for a group of the newly synthesized compounds showed that they possess moderate antibacterial activities against examples of Gram-positive and Gram-negative bacteria.