Aflatoxin B1 is a mycotoxin produced by aspergillus flavus or parasiticus. It is a frequent contaminant of food & feed of human & nonhuman species resulting in severe toxicity & carcinogenicity. Green tea extract has a known anti free radicals, anti-inflammatory and anti-carcinogenic properties. The aim of the present study was to evaluate the ameliorative role of green tea extract against Aflatoxin B1 –induced spleen toxicity in adult albino rats. 72 adult male albino rats were divided into 4 groups & daily administered: Group I (Control Group): (a): negative control, (b) oral dose of olive oil, (c) oral dose of distilled water, Group II: oral dose of green tea extract (200mg/kg) 4 days before starting other administrations & continued till the end of the experiment, Group III: oral dose of Aflatoxin B1 (300ug/kg) for 12 weeks Group IV: oral dose of green tea extract (200mg/kg) and Aflatoxin B1 (300ug/kg) for 12 weeks. After 8 & 12 weeks glutathione peroxidase, catalase & superoxide dismutase were assessed, histopathological & immunohistochemical examination of spleens sections were done. At 8th week, Aflatoxin B1 toxicity group showed significant decrease in glutathione peroxidase, catalase & superoxide dismutase activities (p 0.05), in comparison to the control group with irregular & ill defined outlet of mantel zones. After 12 weeks, few hemosiderin-laden macrophages, neutrophils and strong expression of CD20 were added to previous toxic effects. Green tea treated rats showed a non significant increase in the enzymes activities (p≥ 0.05) after 8 weeks and a significant increase in their levels after 12 weeks (p 0.05). Spleen micro architecture was similar to that of control group with normal CD20 expression after 8 & 12 weeks of the study. However, the number of hemosiderin-laden macrophages was still reduced. It was concluded that green tea extract can offer significant time dependant ameliorative effects for aflatoxin B1-induced spleen toxicity through its anti free radical & immunoprotective properties.