Background and Objective: Wheat (Triticum aestivum L.) is the most needed cereal crop in Egypt and due to the wide gap between wheat production and consumption, it was necessary to discover how to maximize wheat productivity in newly cultivated sandy soil as a way of horizontal expansion. Sandy soil suffers essentially from water stress and weed plants invasion, so this study aimed to compare among three wheat cultivars and three row spacing patterns which could provide a better distribution of plants roots at field to for a better uptake of water and minerals minimize the competition between plants, also the study aimed to compare five different weed control methods to decide the best alternative one. Methodology: Two field experiments were carried out at the experimental farm of faculty of Agriculture, Zagazig university in the winter seasons of 2015/2016 and 2016/2017 in new cultivated sandy soil using three Egyptian cultivars and three row spacings (10, 15 and 20 cm) and five different control methods (check, hand weeding, narrow leaf herbicide, broad leaf herbicide, both narrow and broad leaf herbicide). Results: The results showed significant differences between wheat cultivars in most of root parameters at different soil depths and layers at various growth stages. Also, studied root parameters showed significant variations between row spacing of wheat in favor of 20 cm row spacing. Root parameters significantly varied due to weed control methods. The response of root number density, root length density, root surface area and root dry weight of wheat differed due to weed control methods, soil depths and layers as well as at different wheat growth stages. On the other hand, roots growth rate was significantly affected by cultivars, row spacing and weed control methods. Simple correlation coefficient between wheat grain yield and root growth parameters was not significant, but root number was significant with other root growth parameters. Conclusion: The results of this study indicated that root growth is affected by many of agriculture managements, like row spacing and methods of weed control stated that wheat varieties significantly differed in root patterns and the variation in soil moisture may cause this.