Background: Culture within an organization is very crucial; it plays an important role in the hospital and is considered a main predictor of the organizational commitment; subsequently, the organizational changes can be managed successfully. The present study aimed to assess the organizational culture perception as a predictor of organizational commitment and attitudes toward organizational change among nurses at Al-Ahrar Teaching Hospital, Sharkia Governorate, Egypt. For this research, a descriptive correlation design was used. Subjects and methods: A simple random sample of 221 nurses were chosen from the above mentioned setting. To fulfill the purpose of this study three tools were used in data collection, organizational culture inventory, organizational commitment scale, and attitude toward organizational change instrument. Results showed that 91.90 % of nurses had a positive perception of the constructive culture. On the other hand, 76.9% and 74.7% of them had negative perceptions of aggressive defensive and passive defensive cultures, respectively. Likewise, 57.50% of nurses had high level of organizational commitment. Moreover, 76% of studied nurses had positive perception of attitude toward change. Furthermore, organizational commitment was highly statistically significantly and positively correlated to all types of the organizational culture, where p-value < 0.01. Besides, there were highly statistically significant and positive correlations between attitude toward organizational change as regards all types of the organizational culture and organizational commitment, where p-value < 0.01. Conclusion: The constructive culture was the most influential and predictor type of the organizational commitment; however, the passive defensive culture was the most influential and predictor type of nurses' attitudes toward organizational change. Recommendation: The nurse managers should schedule regular meetings with their staff nurses to define and communicate the organizational culture before the initiation of the change process.