The aim of this study was to investigate the total phenolic compounds and anti-obesity activity of both (Carthamus tinctorius L.) seeds and its extract. HPLC analysis indicated the presence of some polyphenolic compounds which, responsible for their therapeutic potential. Treatment of aqueous extract significantly reduced the elevated serum levels of Glucose, Lipase, Aspartate amino transferase (AST) and Alanine amino transferase (ALT). Twenty present of safflower seeds reduced level of Uric acid, Creatinine and Urea nitrogen. A significant increase in HDL-c but decrease in LDL-c level was observed compared with control group. In histopathological study aqueous extract showed minimal to mild multifocal and diffuse granular degeneration and mild to moderate lobular disarray compared to control group.