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Purification and characterization of two chitinases from Streptomyces albovinaceus S-22
Faculty
Science
Year:
2000
Type of Publication:
Article
Pages:
87-89
Authors:
Ezzat, SM, Ghaly, MF, El-Sayed, ESA, Mansour, M, El-Bohey, MA
DOI:
10.1023/A:1008926214392
Journal:
WORLD JOURNAL OF MICROBIOLOGY \& BIOTECHNOLOGY KLUWER ACADEMIC PUBL
Volume:
16
Research Area:
Biotechnology \& Applied Microbiology
ISSN
ISI:000085316400017
Keywords :
Purification , characterization , , chitinases from Streptomyces albovinaceus
Abstract:
Two chitinases, A and B, were purified from the culture supernatant of Streptomyces albovinaceus S-22 by ammonium sulphate fraction (80\%) and Sephadex G-200 gel filtration. Both enzymes had molecular weights estimated to be 43 and 45 kDa by SDS polyacrylamide gel electrophoresis. The enzymes were active at 40 degrees C and pH 5.6 after 120 min, and stable at temperatures below 40 degrees C in the absence of chitin. The purified enzyme had potential for cell wall lysis of fungal pathogenesis tested.
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