Evaluating trends in water quality indicators is a crucial issue in integrated water resource management in any country. In this study eight chemical and physical water quality indicators were analysed in seven river profiles in the River Laborec in eastern Slovakia. The analysed water quality parameters were biochemical oxygen demand (BOD5), chemical oxygen demand (CODCr), pH, temperature (t), ammonium nitrogen (NH4 +-N), nitrite nitrogen (NO2 --N), nitrate nitrogen (NO3 --N), and total phosphorus (TP). Data from the monitored indicators were provided by the Košice branch of the Slovakian Water Management Company, over a period of 15 years from 1999 to 2013. Mann–Kendall non-parametric statistical test was used for the trend analysis. Biochemical and chemical oxygen demand, ammonium and nitrite nitrogen content exhibit decreasing trends in the River Laborec. Decreasing agricultural activity in the area has had a significant impact on the trends in these parameters. However, NO2 --N was the significant parameter of water quality because it mostly exceeds the limit value set in Slovak legislation, Regulation No. 269/2010 Coll. In addition, water temperature revealed an increasing trend which could be caused by global increase in air temperature. These results indicate that human activity significantly impacts the water quality.