Zagazig University Digital Repository
Home
Thesis & Publications
All Contents
Publications
Thesis
Graduation Projects
Research Area
Research Area Reports
Search by Research Area
Universities Thesis
ACADEMIC Links
ACADEMIC RESEARCH
Zagazig University Authors
Africa Research Statistics
Google Scholar
Research Gate
Researcher ID
CrossRef
Induction of apoptosis in cancer cells by NiZn ferrite nanoparticles through mitochondrial cytochrome C release
Faculty
Pharmacy
Year:
2013
Type of Publication:
Article
Pages:
4115-4130
Authors:
Al-Qubaisi, Mothanna Sadiq, Rasedee, Abdullah, Flaifel, Moayad Husein, Ahmad, Sahrim Hj, Hussein-Al-Ali, Samer, Hussein, Mohd Zobir, Zainal, Zulkarnain, Alhassan, Fatah H, Taufiq-Yap, Yun H, Eid, Eltayeb E. M, Arbab, Ismail Adam, Al-Asbahi, Bandar A, Webster, Thomas J, El Zowalaty, Mohamed Ezzat
DOI:
10.2147/IJN.S50061
Journal:
INTERNATIONAL JOURNAL OF NANOMEDICINE DOVE MEDICAL PRESS LTD
Volume:
8
Research Area:
Science \& Technology - Other Topics; Pharmacology \& Pharmacy
ISSN
ISI:000326386000001
Keywords :
NiZn ferrite nanoparticles, cancer cells, reactive oxygen species, cytochrome C, mitochondrial membrane potential, p53
Abstract:
The long-term objective of the present study was to determine the ability of NiZn ferrite nanoparticles to kill cancer cells. NiZn ferrite nanoparticle suspensions were found to have an average hydrodynamic diameter, polydispersity index, and zeta potential of 254.2 +/- 29.8 nm, 0.524 +/- 0.013, and -60 +/- 14 mV, respectively. We showed that NiZn ferrite nanoparticles had selective toxicity towards MCF-7, HepG2, and HT29 cells, with a lesser effect on normal MCF 10A cells. The quantity of Bcl-2, Bax, p53, and cytochrome C in the cell lines mentioned above was determined by colorimetric methods in order to clarify the mechanism of action of NiZn ferrite nanoparticles in the killing of cancer cells. Our results indicate that NiZn ferrite nanoparticles promote apoptosis in cancer cells via caspase-3 and caspase-9, downregulation of Bcl-2, and upregulation of Bax and p53, with cytochrome C translocation. There was a concomitant collapse of the mitochondrial membrane potential in these cancer cells when treated with NiZn ferrite nanoparticles. This study shows that NiZn ferrite nanoparticles induce glutathione depletion in cancer cells, which results in increased production of reactive oxygen species and eventually, death of cancer cells.
Online
PDF
جامعة المنصورة
جامعة الاسكندرية
جامعة القاهرة
جامعة سوهاج
جامعة الفيوم
جامعة بنها
جامعة دمياط
جامعة بورسعيد
جامعة حلوان
جامعة السويس
شراقوة
جامعة المنيا
جامعة دمنهور
جامعة المنوفية
جامعة أسوان
جامعة جنوب الوادى
جامعة قناة السويس
جامعة عين شمس
جامعة أسيوط
جامعة كفر الشيخ
جامعة السادات
جامعة طنطا
جامعة بنى سويف