The current investigation was carried out during 2015 and 2016 seasons on El-mamoura guava fruits (Psidium guajava L.) to study the effect of calcium nitrate and chitosan coating treatments on the physico-chemical characteristics of the fruits during cold storage period. All treatments prevented fruit decay compared with control which lost 26.14 % (average of the two seasons) of its fruits. Guava fruits didn’t lost any weight during the first two weeks of cold storage in both seasons and after 43 days the least weight loss percentages were for the treatments of Calc. Nit. 4% + Chitosan 0.5% in the first season and Calc. Nit. 2% + Chitosan 1% in the second season. The above treatment of Calc. Nit. 2% + Chitosan 1% had the highest significant average of V.C and gave the highest firmness values in both seasons and its fruits had the highest citric acid content in the last interval of the first season. All treatments maintained the TSS content in guava fruits during cold storage period and the treatment of Calc. Nit. 4% + Chitosan 0.5% had the highest significant average value in both seasons.