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In vitro evaluation of various antimicrobials against field mycoplasma gallisepticum and mycoplasma synoviae isolates in Egypt
Faculty
Veterinary Medicine
Year:
2019
Type of Publication:
ZU Hosted
Pages:
Authors:
Naglaa Fathy Said Awad
Staff Zu Site
Abstract In Staff Site
Journal:
International Journal of Fertility and Sterility Oxford University Press
Volume:
Keywords :
, vitro evaluation , various antimicrobials against field
Abstract:
Among many avian mycoplasmas, Mycoplasma gallisepticum (MG) and Mycoplasma synoviae (MS) are recognized as the main etiological agents of respiratory diseases and infectious synovitis in chickens and turkeys causing tremendous economic losses worldwide. Therefore, proper treatment is promoted for the control of these diseases. This study was the first in Egypt to evaluate the in vitro efficacy of various antimicrobials against field MG and MS isolates recovered from chicken and turkey flocks using both conventional broth microdilution and quantitative real-time polymerase chain reaction assays. Totally, 47 mycoplasma isolates were recovered from 160 collected tracheal samples (29.4%). Of these, 44 MG (27.5%) and 3 MS (1.9%) were identified using conventional and molecular assays. The in vitro susceptibilities of 4 representative mycoplasma field isolates (3 MG and one MS) to 8 antibiotics and 4 essential oils were investigated. The tested isolates showed various susceptibilities to tested antimicrobials. Toldin CRD, followed by clove, cumin, and cinnamon oils were effective against both MG and MS clinical isolates with minimum inhibitory concentration (MIC) values ranging from 0.49 to 15.63 μg/mL. Similarly, tylvalosin was the most active antibiotic against MG and MS isolates with the lowest MIC values (0.015 to 0.03 μg/mL). DNA loads of both MG mgc2 and MS vlhA genes were markedly decreased upon treatment with majority of the tested antimicrobials confirming their effectiveness as was also evaluated by conventional MIC results. In conclusion, Toldin CRD and tylvalosin were found to be the most effective antimicrobials in this study. This finding highlights the importance of using these antimicrobials in controlling mycoplasma infections in chickens and turkeys.
Author Related Publications
Naglaa Fathy Said Awad, "Phylogenetic Analysis of Infectious Bronchitis Viruses Currently Circulating in the Egyptian Field", Faculty of Veterinary Medicine, Zagazig University, 2019
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Naglaa Fathy Said Awad, "Genetic characterization of rabbit hemorrhagic disease virus from naturallyinfected rabbits in Sharkia governorate, Egypt", Egyptian Society of Virology, 2018
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Naglaa Fathy Said Awad, "Coexistence of Virulence and Antibiotic Resistance Genes in Pasteurella multocida Isolated from Diseased Rabbits", Faculty of Veterinary Medicine, Zagazig University, 2019
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Naglaa Fathy Said Awad, "Molecular Diagnosis of Persistently Very Virulent Infectious Bursal Disease Virus at Sharkia Governorate, Egypt", C M B Association, 2019
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Naglaa Fathy Said Awad, "Impact of single and mixed infections with Escherichia coli and Mycoplasma gallisepticum on Newcastle disease virus vaccine performance in broiler chickens: an in vivo perspective", Wiley-Blackwell on behalf of the Society for Applied Microbiology, 2019
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Department Related Publications
Amal Anis Mahdi Eid, "): Anticoccidial Efficacy of Artermisia cina and its extracts in Broiler Chickens. Zag. Vet. J. Vol. 32(2), 163-167.", لايوجد, 1900
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Ibrahiem Abdelrahman Ibrahiem Ghanem, ":تقييم بعض اللقاحات الحيه المستخدمه لمرض الجمبورو في الحقل", لايوجد, 1900
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Ibrahiem Abdelrahman Ibrahiem Ghanem, "عدوي فيروس الجدري في بعض الطيور بمحافظه الشرقيه", لايوجد, 1900
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Ibrahiem Abdelrahman Ibrahiem Ghanem, "ملاحظات علي ظاهرة التقزم في الدجاج البلدي-دراسات اكلينيكيه بيوكيميائيه", لايوجد, 1900
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Ibrahiem Abdelrahman Ibrahiem Ghanem, "العلاقه الداخليه بين غدة هاردر وحويصله فابريشيا للاستجابه المناعيه", لايوجد, 1900
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