This study aimed to utilize Pseudomonas fluorescens metabolites as biopriming agent for induction systemic resistance against Fusarium wilt in tomato plants. Two groups of tomato seeds were used, the first was kept dry and the second was primed with Pseudomonas fluorescens metabolites and planted under Fusarium infection conditions. The primed group can withstand the infection, while the non-primed plant cannot withstand the infection. The success of the primed group to fight the infection is attributed to induction of systemic resistance. This induction appeared in the increase in the signal transduction molecules. These signal molecules induced the activity of the defense enzymes