Sphingolipid-induced cell death in Arabidopsis is negatively regulated by the papain-like cysteine protease RD21

Faculty Agriculture Year: 2019
Type of Publication: ZU Hosted Pages: 20239-20262
Authors:
Journal: Multimedia Tools and Applications Elsevier Volume: 14
Keywords : Sphingolipid-induced cell death , Arabidopsis , negatively regulated    
Abstract:
It is now well established that sphingoid Long Chain Bases (LCBs) are crucial mediators of programmed cell death. In plants, the mycotoxin fumonisin B1 (FB1) produced by the necrotrophic fungus Fusarium moniliforme disrupts the sphingolipid biosynthesis pathway by inhibiting the ceramide synthase leading to an increase in the amount of phytosphingosine (PHS) and dihydrosphingosine (DHS), the two major LCBs in Arabidopsis thaliana. To date, the signaling pathway involved in FB1-induced cell death remains largely uncharacterized. It is also well acknowledged that plant proteases such as papain-like cysteine protease are largely involved in plant immunity. Here, we show that the papain-like cysteine protease RD21 (responsive-to-desiccation-21) is activated in response to PHS and FB1 in Arabidopsis cultured cells and leaves, respectively. Using two allelic null mutants of RD21, and two different PCD bioassays, we demonstrate that the protein acts as a negative regulator of FB1-induced cell death in Arabidopsis.
   
     
 
       

Author Related Publications

  • Khaled Youssef Kamal Mostafa , "Evaluating deficit irrigation scheduling strategies to improve yield and water productivity of maize in arid environment using simulation", ScienceDirect, 2022 More
  • Khaled Youssef Kamal Mostafa , "Embedding Arabidopsis Plant Cell Suspensions in Low-Melting Agarose Facilitates Altered Gravity Studies", Springer, 2017 More
  • Khaled Youssef Kamal Mostafa , "Cell cycle acceleration and changes in essential nuclear functions induced by simulated microgravity in a synchronized Arabidopsis cell culture", Wiley Online Library, 2018 More
  • Khaled Youssef Kamal Mostafa , "Simulated microgravity, Mars gravity, and 2g hypergravity affect cell cycle regulation, ribosome biogenesis, and epigenetics in Arabidopsis cell cultures", Nature group, 2018 More
  • Khaled Youssef Kamal Mostafa , "Differential transcriptional profile through cell cycle progression in Arabidopsis cultures under simulated microgravity", elsevier, 2019 More

Department Related Publications

  • Hassan Odaa Awad Odaa, "Update, Conclusions, and Recommendations for Sustainability of the Agricultural Environment in Egypt: The Soil–Water–Food Nexus", Springer Nature, 2018 More
  • Fares Suleiman Mohammed Juma, "INNOVATION OF A COMPUTERIZED IRRIGATION MANAGEMENT MODEL BASED ON WATER BALANCE EQUATION IN INTERCROPPED WHEAT IN OLIVE FIELD", مجلة كلية الزراعه جامعة الزقازيق, 2014 More
  • Abdelhamid Hassan Salem Abotaleb, "Some Stability Parameters in Sunflower (Helianthus annuus L.) Genotypes at Various Applications", الجمعية المصرية لعلوم المحاصيل, 2013 More
  • Khaled Youssef Kamal Mostafa , "Evaluation of growth and nutritional value of Brassica microgreens grown under red, blue and green LEDs combinations", Wiley online Library, 2020 More
  • Asmaa Abdelsalam Mohamed alsaied, "Evaluation of growth and nutritional value of Brassica microgreens grown under red, blue and green LEDs combinations", Wiley online Library, 2020 More
Tweet