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Oxidative Stress Infuences Pseudomonas aeruginosa Susceptibility to Antibiotics and Reduces Its Pathogenesis in Host
Faculty
Pharmacy
Year:
2020
Type of Publication:
ZU Hosted
Pages:
Authors:
Mo'men Mahmoud Ezzelarab Abdelaziz Metwally Askoura
Staff Zu Site
Abstract In Staff Site
Journal:
مجلة بحوث التربية الشاملة Springer
Volume:
Keywords :
Oxidative Stress Infuences Pseudomonas aeruginosa Susceptibility
Abstract:
Pseudomonas aeruginosa is a Gram-negative opportunistic pathogen that causes serious infections in humans, notably cystic fbrosis. P. aeruginosa faces various stresses such as oxidative stress either in the environment or within the host during infection. In the present study, the infuence of oxidative stress on both Pseudomonas antibiotic susceptibility and host pathogenesis was characterized. Prior exposure to H2O2 signifcantly altered P. aeruginosa susceptibility to tested antibiotics; colistin, ciprofoxacin, tobramycin, and ceftazidime. The minimum inhibitory concentrations (MICs) of tested antibiotics either increased or decreased following H2O2 exposure. Importantly, RT-qPCR revealed that expression of quorum sensing genes, that regulate virulence factors production in P. aeruginosa, was signifcantly higher in unstressed relative to H2O2-stressed cells. The impact of P. aeruginosa exposure to oxidative stress by H2O2 on bacterial pathogenesis was investigated using in vivo mice infection model. Interestingly, exposure to oxidative stress markedly reduces P. aeruginosa pathogenesis in mice. Unstressed P. aeruginosa was able to kill more mice as compared to H2O2-stressed bacteria. In addition, body weight of mice infected with unstressed P. aeruginosa was lower than that of mice inoculated with stressed bacteria. Isolated organs (spleen, liver, and kidney) from mice infected with unstressed bacteria exhibited increased weight as well as bacterial load in comparison with mice infected with stressed bacteria. In summary, current data highlight the impact of oxidative stress on P. aeruginosa antibiotic susceptibility as well as host pathogenesis. These fndings could be helpful in treatment of infections caused by this important pathogen.
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Mo'men Mahmoud Ezzelarab Abdelaziz Metwally Askoura, "Alteration of Salmonella enterica Virulence and Host Pathogenesis through Targeting sdiA by Using the CRISPR-Cas9 System", MDPI, 2021
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Mo'men Mahmoud Ezzelarab Abdelaziz Metwally Askoura, "efflux pump inhibitors epis as new antimicrobila agents against peseudomnas aeruginosa", libyan journal of microbiology, 2011
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Mo'men Mahmoud Ezzelarab Abdelaziz Metwally Askoura, "listeria monocytogenes internalins: a diverse proteinfam invoved in virulence", the libyan journal of infectious disases, 2010
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Mo'men Mahmoud Ezzelarab Abdelaziz Metwally Askoura, "The Campylobacter jejuni Ferric Uptake Regulator Promotes Acid Survival and Cross-Protection against Oxidative Stress.", American Society for Microbiology, 2016
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Mo'men Mahmoud Ezzelarab Abdelaziz Metwally Askoura, "In Vitro Study on the Pathogenicity of Proteus Species Isolated", لايوجد, 1900
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Mona Abdelmoniem Elsayed Mahmmoud , "The value of peritoneal lavage in peritonitis", لايوجد, 1900
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