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Susceptibility to trypsinolysis of esterified milk proteins
Faculty
Agriculture
Year:
2001
Type of Publication:
Article
Pages:
263-271
Authors:
SITOHY, M, CHOBERT, JM, HAERTLE, T
DOI:
10.1016/S0141-8130(01)00125-8
Journal:
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES ELSEVIER SCIENCE BV
Volume:
28
Research Area:
Biochemistry \& Molecular Biology
ISSN
ISI:000167778400001
Keywords :
esterification, trypsin, milk proteins
Abstract:
Methyl-, ethyl- and propyl-esters of beta -lactoglobulin. alpha -lactalbumin and beta -casein were prepared and then hydrolyzed with trypsin in various conditions. Resulting hydrolysates were analysed by SDS electrophoresis and RP-HPLC. The degree of hydrolysis of esterified samples was generally lower than those of the non-modified proteins. The highest degrees of hydrolysis were obtained at pH 7-8 with native and esterified protein samples. beta -Lactoglobulin propyl ester and beta -casein methyl ester yielded the lowest degrees of hydrolysis. Ethyl- and propyl-esters of beta -casein showed high resistance towards tryptic attack, even after 20 h of hydrolysis. SDS electrophoretic patterns of tryptic hydrolysates of native proteins showed bands corresponding to low molecular weights. Tryptic hydrolysates of esterified proteins showed bands with higher sizes. RP-HPLC profiles of tryptic hydrolysates of esterified samples showed peaks with longer elution times than those obtained with native proteins, indicating the presence of more hydrophobic peptide populations. A peptic pre-treatment improved tryptic action on esterified proteins. It resulted in a better resolution of RP-HPLC profiles and in a complete disappearance of the protein after 20 h tryptic hydrolysis. (C) 2001 Elsevier Science B.V. All rights reserved.
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