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Improved LC methods for the determination of diosmin and/or hesperidin in plant extracts and pharmaceutical formulations
Faculty
Pharmacy
Year:
2001
Type of Publication:
Article
Pages:
539-545
Authors:
El-Shafae, AM, El-Domiaty, MM
DOI:
10.1016/S0731-7085(01)00476-9
Journal:
JOURNAL OF PHARMACEUTICAL AND BIOMEDICAL ANALYSIS PERGAMON-ELSEVIER SCIENCE LTD
Volume:
26
Research Area:
Chemistry; Pharmacology \& Pharmacy
ISSN
ISI:000171735500004
Keywords :
high-performance liquid chromatography diosmin and hesperidin, buchu leaves, pharmaceutical formulations
Abstract:
Two improved. simple. selective and sensitive reversed-phase HPLC methods were developed for the determination of diosmin and hesperidin simultaneously or separately, using external or internal standard techniques, respectively. The methods were applied for determination of the diosmin and/or hesperidin in Buchu leaves and pharmaceutical dosage forms (three tablets). The methods involved very simple efficient extraction procedures, the use of a LiChrosorb RP-18 column, a mixture of methanol-water (60:40. v/v) as a mobile phase in an isocratic mode at a flow rate of 1.5 ml, min and UV detection at 345 nm. In all HPLC analysis the relative standard deviation did not exceed 1.9\%. As low as 50 and 25 ng (per injection) could be detected in less than 6 min with a high degree of accuracy. This is the first report for the determination of the two flavonoids simultaneously in Buchu leaf and tablets containing them. (C) 2001 Elsevier Science B.V. All rights reserved.
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