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Thymoquinone suppresses expression of inducible nitric oxide synthase in rat macrophages
Faculty
Veterinary Medicine
Year:
2002
Type of Publication:
Article
Pages:
1603-1611
Authors:
Shimizu, Y, Takewaki, T, El-Mahmoudy, A, Matsuyama, H, Borgan, MA, El-Sayed, MG, Minamoto, N
DOI:
10.1016/S1567-5769(02)00139-X
Journal:
INTERNATIONAL IMMUNOPHARMACOLOGY ELSEVIER SCIENCE BV
Volume:
2
Research Area:
Immunology; Pharmacology \& Pharmacy
ISSN
ISI:000179095700010
Keywords :
inducible nitric oxide synthase, macrophage, nitric oxide, thymoquinone
Abstract:
The objective of the present study was to determine the immumomodulatory role of thymoquinone (TQ) regarding its effect on the production of nitric oxide (NO) by rat peritoneal macrophages. Under certain conditions, macrophages and certain other cells can produce high concentrations of NO from its precursor L-arginine via inducible nitric oxide synthase (iNOS) pathway. TQ has been established as the major component of the oil extracted from Nigella sativa plant seeds, which is being used frequently in herbal medicine. TQ (IC50 1.4-2.76 muM) dose- and time-dependently reduced nitrite production, a parameter for NO synthesis, in supernatants of lipopolysaccharide (LPS)-stimulated (5 mug/ml) macrophages without affecting the cell viability. The protein level of iNOS in peritoneal macrophages was also decreased by TQ in a concentration-dependent manner. In addition, TQ inhibited the increase in iNOS mRNA expression induced by LPS indicated by reverse transcription-polymerase chain reaction (RT-PCR). These inhibitory effects of TQ were confirmed by immunofluorescence staining of iNOS in macrophages which showed decreased immunoreactivity for iNOS after treatment with TQ if compared with the control LPS-stimulated cells. These results suggest that TQ suppresses the production of NO by macrophages; an effect which may be useful in ameliorating the inflammatory and autoinumme conditions. (C) 2002 Elsevier Science B.V. All rights reserved.
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