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Immunolocalization of an enterotoxic glycoprotein exoantigen on the secretory organelles of Cryptosporidium parvum sporozoites
Faculty
Science
Year:
2004
Type of Publication:
Article
Pages:
225-230
Authors:
El-Shewy, KA, Eid, RA
Journal:
PARASITE-JOURNAL DE LA SOCIETE FRANCAISE DE PARASITOLOGIE PRINCEPS EDITIONS
Volume:
11
Research Area:
Parasitology
ISSN
ISI:000222395600016
Keywords :
Cryptosporidium parvum, coproantigen, 18-20 kDa glycoprotein, exoantigen, immunolocalization
Abstract:
In this study, the fine ultrastructures of the secretary organelles of C. parvum sporozoites were demonstrated using transmission electron microscopy (TEM). Meanwhile, a previously identified enterotoxic 18-20 kDa copro-antigen (18-20 kDc CCA), associated with cryptosporidiosis in both human and calves, was isolated and immunolocalized on C. parvum sporozoites. Using immunoelectron microscopy and anti-18-20 kDa monospecific antibody demonstrated marked existence of the 18-20 kDa CCA on the apical organelles and at the trilaminar pellicles. An anterior extrusion of this protein was demonstrated around the excysted and released sporozoites. However, non excysted sporozoites did not show this protein. Affinity blotting, with biotinylated jacalin, demonstrated the O-linked oligosaccharide moiety of this protein. The potential role of this protein in the host cell invasion and/or gliding motility remains unelucidated. However, its enterotoxicity, location and secretary nature suggest that it may be a target for neutralization or invasion inhibition of Cryptosporidium.
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