Zagazig University Digital Repository
Home
Thesis & Publications
All Contents
Publications
Thesis
Graduation Projects
Research Area
Research Area Reports
Search by Research Area
Universities Thesis
ACADEMIC Links
ACADEMIC RESEARCH
Zagazig University Authors
Africa Research Statistics
Google Scholar
Research Gate
Researcher ID
CrossRef
Successful abrogation by thymoquinone against induction of diabetes mellitus with streptozotocin via nitric oxide inhibitory mechanism
Faculty
Veterinary Medicine
Year:
2005
Type of Publication:
Article
Pages:
195-207
Authors:
El-Sayed, M, Shimizu, Y, Shiina, T, Takewaki, T, El-Mahmoudy, A, Matsuyama, H
DOI:
10.1016/j.intimp.2004.09.001
Journal:
INTERNATIONAL IMMUNOPHARMACOLOGY ELSEVIER SCIENCE BV
Volume:
5
Research Area:
Immunology; Pharmacology \& Pharmacy
ISSN
ISI:000226615400029
Keywords :
diabetes, macrophage, nitric oxide, streptozotocin, thymoquinone
Abstract:
Nitric oxide (NO) is involved in the destruction of beta-cells during the development of type I diabetes mellitus (DM). We demonstrated the possibility of rescuing beta-cells by intervention with thymoquinone (TQ) using streptozotocin (STZ) rat diabetic model. The hyperglycemic and hypoinsulinemic responses to STZ were significantly abrogated in rats cotreated with TQ and this abrogating effect has persisted for 1 month after stopping of TQ treatment. Unlike observations recorded after diabetic chronicity of 1 month, where there was a significant reduction of both serum and pancreatic nitrites, a significant increase in both nitrites was observed within the first 3 days in STZ rats. with or without lipopolysaccharide (LPS) stimulation, compared with controls and the TQ-cotreated. In vitro production of nitrite was significantly higher by 3-day-diabetic macrophages with or without stimulation compared to control or TQ-treated ones. However, 1-month-diabetic macrophages showed insignificant decrease of nitrite which turned significant upon stimulation. TQ has no effect on either IkB degradation or NF-kB activation; however, it significantly inhibited both p44/42 and p38 mitogen-activated protein kinases (MAPKs) which contribute to the transcriptional machinery of inducible nitric oxide synthase and NO production. These data emphasize the protective value of TQ against development of type I DM via NO inhibitory pathway. (C) 2004 Elsevier B.V. All rights reserved.
Online
PDF
جامعة المنصورة
جامعة الاسكندرية
جامعة القاهرة
جامعة سوهاج
جامعة الفيوم
جامعة بنها
جامعة دمياط
جامعة بورسعيد
جامعة حلوان
جامعة السويس
شراقوة
جامعة المنيا
جامعة دمنهور
جامعة المنوفية
جامعة أسوان
جامعة جنوب الوادى
جامعة قناة السويس
جامعة عين شمس
جامعة أسيوط
جامعة كفر الشيخ
جامعة السادات
جامعة طنطا
جامعة بنى سويف