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Screening of bioactive lipids and radical scavenging potential of some solanaceae plants
Faculty
Agriculture
Year:
2007
Type of Publication:
Article
Pages:
885-890
Authors:
Ramadan, Mohamed Fawzy, El-Shamy, Hesham, Zayed, Rawya
DOI:
10.1016/j.foodchem.2006.09.040
Journal:
FOOD CHEMISTRY ELSEVIER SCI LTD
Volume:
103
Research Area:
Chemistry; Food Science \& Technology; Nutrition \& Dietetics
ISSN
ISI:000245411500028
Keywords :
chromatographic analysis, fatty acids, lipid-soluble bioactives, radical scavenging activity, DPPH, solanaceae
Abstract:
Gas-liquid chromatography (GLC) and normal-phase high performance liquid chromatography (HPLC) were used to analyse fatty acids and fat-soluble bioactives of the seeds of four Datura species (D. stramonium, D. metel, D. tatula, D. innoxia) and two Ryoscyamus species (H. muticus, H. niger). The amounts of n-hexane extract were found to be between 5.50\% and 12.6\%. The major fatty acid was linoleic acid followed by oleic, palmitic and stearic acids. The crude n-hexane extract was characterised by a relatively high amount of phytosterols, wherein the sterol markers were stigmasterol, beta-sitosterol, lanosterol, Delta 5-avenasterol and sitostanol. In all plant extracts gamma-tocopherol was the major component present accounting for more than 80\% of total tocopherols detected. When different extracts were compared for their radical scavenging activity (RSA) toward the stable 2,2-diphenyl-l-picrylhydrazyl (DPPH) radical, D. Innoxia exhibited a stronger RSA. (c) 2006 Elsevier Ltd. All rights reserved.
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