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Isolation and characterization of a lectin with antifungal activity from Egyptian Pisum sativum seeds
Faculty
Agriculture
Year:
2007
Type of Publication:
Article
Pages:
971-979
Authors:
Sitohy, Mahmoud, Doheim, Mahmoud, Badr, Haitham
DOI:
10.1016/j.foodchem.2007.01.026
Journal:
FOOD CHEMISTRY ELSEVIER SCI LTD
Volume:
104
Research Area:
Chemistry; Food Science \& Technology; Nutrition \& Dietetics
ISSN
ISI:000247275700012
Keywords :
Pisum sativum, Leguminoseae, purification, characterization, mannose binding lectin, antifungal, hemagglutination activity
Abstract:
A plant lectin isolated in its pure state from the Egyptian seeds of Pisum sativum (PSL) produced two bands in SDS-PAGE (5.53 and 19.3 kDa; i.e. alpha and beta chain) but one peak by gel filtration chromatography on Sephadex G-100, corresponding to 50 kDa, i.e., a dimeric structure of two monomers, each consisting of one alpha and one beta subunit. PSL is a glycoprotein bound with glucose (2 mol/mol of protein) and stabilized by 2 atoms of each of Ca2+ and Mn2+ per molecule of protein. It highly agglutinated human, rabbit and rat erythrocytes but weakly agglutinated chicken erythrocytes, while no agglutination occurred with sheep erythrocytes. Hemagglutination was markedly affected by acidic pH, but was heat stable below 60 degrees C for 30 min. Among the various tested sugars, PSL agglutination was most inhibited by mannose. PSL is rich in hydroxyl amino acids while totally lacking sulfur amino acids. PSL inhibited the growth of Aspergiffils flavus, Trichoderma viride and Fusarium oxysporum. (c) 2007 Elsevier Ltd. All rights reserved.
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جامعة الاسكندرية
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