Zagazig University Digital Repository
Home
Thesis & Publications
All Contents
Publications
Thesis
Graduation Projects
Research Area
Research Area Reports
Search by Research Area
Universities Thesis
ACADEMIC Links
ACADEMIC RESEARCH
Zagazig University Authors
Africa Research Statistics
Google Scholar
Research Gate
Researcher ID
CrossRef
Determination of oxomemazine in human plasma by capillary LC-ESI-MS
Faculty
Science
Year:
2007
Type of Publication:
Article
Pages:
393-403
Authors:
Elmosallamy, M. A. F, Saber, A. L, Wilson, S. R, Lundanes, E, Greibrokk, T
DOI:
10.1080/10826070601084860
Journal:
JOURNAL OF LIQUID CHROMATOGRAPHY \& RELATED TECHNOLOGIES TAYLOR \& FRANCIS INC
Volume:
30
Research Area:
Biochemistry \& Molecular Biology; Chemistry
ISSN
ISI:000243121900006
Keywords :
capillary liquid chromatography, column switching, oxomemazine, liquid-liquid extraction, mass spectrometry
Abstract:
A method based on on-line solid phased extraction capillary liquid chromatography-electrospray ionization-mass spectrometry (SPE-capLC-ESI-MS) has been developed for the determination of oxomemazine in human plasma. Prior to injection, 0.5 mL of plasma spiked with metronidazole (internal standard) was mixed with ammonium formate buffer and methyl orange, which served as an ion pair reagent for effective chloroform liquid - liquid extraction. The employment of methyl orange as an ion pair reagent doubled the extraction efficiency, as compared to not using methyl orange. In preliminary experiments, conventional LC-UV instrumentation was employed. However, it was found that employing a capillary column with an inner diameter of 0.3 mm increased the sensitivity by a factor of similar to 100, when injecting the same mass of analyte. Incorporating an easily automated reversed phase column switching system with SPE made it possible to inject up to 100 mL of solution, and the total analysis time was 5 minutes. The method was validated in the range 3 to 30 ng/mL oxomemazine, yielding a correlation coefficient of 0.99 (r(2)). The within-assay and between-assay precisions were between 6.7 and 12\% and 6.8 and 7.4\%, respectively. The method was used to determine the amount of oxomemazine in a healthy female 20 hours after an lintake of 1 teaspoon (approximately 1 mL) of the cough syrup Toplexil (R), which contains 0.033 g oxomemazine per 100 mL syrup. Oxomemazine was detected, and the concentration was calculated to 2.0 ng/mL plasma.
Online
PDF
جامعة المنصورة
جامعة الاسكندرية
جامعة القاهرة
جامعة سوهاج
جامعة الفيوم
جامعة بنها
جامعة دمياط
جامعة بورسعيد
جامعة حلوان
جامعة السويس
شراقوة
جامعة المنيا
جامعة دمنهور
جامعة المنوفية
جامعة أسوان
جامعة جنوب الوادى
جامعة قناة السويس
جامعة عين شمس
جامعة أسيوط
جامعة كفر الشيخ
جامعة السادات
جامعة طنطا
جامعة بنى سويف