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Antiviral activity of esterified alpha-lactalbumin and beta-lactoglobulin against herpes simplex virus type 1. Comparison with the effect of acyclovir and L-polylysines
Faculty
Agriculture
Year:
2007
Type of Publication:
Article
Pages:
10214-10220
Authors:
Sitohy, Mahmoud, Chobert, Jean-Marc, Haertle, Thomas, Billaudel, Sylviane
DOI:
10.1021/jf0724421
Journal:
JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY AMER CHEMICAL SOC
Volume:
55
Research Area:
Agriculture; Chemistry; Food Science \& Technology
ISSN
ISI:000251477100021
Keywords :
whey proteins, alpha-lactalbumin, beta-lactoglobulin, herpes simplex virus, acyclovir, L-polylysine
Abstract:
The antiviral activity of methylated alpha-lactalburnin (Met-ALA), methylated and ethylated beta-lactoglobulins (Met- and Et-BLG) was evaluated against acyclovir (ACV)-sensitive and -resistant strains of herpes simplex virus type 1 (HSV-1) and compared to that of ACV and L-polylysines (4-15 kDa) using fixed or suspended Vero cell lines. Esterified whey proteins and their peptic hydrolyzates displayed protective action against HSV-1, which was relatively lower than that induced by ACV or L-pOlylysines. The higher activity Of L-polylysines was maintained against an ACV-resistant strain of HSV-1, whereas ACV lost much of its activity. The mean 50\% inhibitory concentration (IC50) was about 0.8-0.9 mu g/mL for L-polylysines against ACV-sensitive and -resistant strains of HSV-1 when using two concentrations of virus (50\% and 100\% cytopathic effect, CPE). The IC50 values of ACV against the sensitive strain of HSV-1 were 3 and 15 mu g/mL when using the low and high concentrations of virus, respectively. When using 50\% CPE, IC50 values for esterified whey proteins ranged from 20 to 95,mu g/mL, depending on the nature of the ester group, the degree of esterification, and the nature of the protein. Using the real-time PCR technique, it was shown that Met-ALA inhibited HSV-1 replication.
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