Phenotypic And Molecular Studies on Stem Cells

Mesenchymal stem cells have been shown to exist in cord blood. Although MSCs have been described by a subset of surface antigens after expansion. little is known about the cell surfacephenotype of undifferentiated ivlSCs. Human umbilical cord bloodstem cells able to di ffercntiatc into hepatocytes in vitro.The present study is conducted to obtain an HSC-enrichedpopulation (lineage-negative and positive CD34 cells) from theUCB and characterize their proliferation and differentiationcapacities under some physiological conditions available and easyfor application in both basic and clinical research.The present study showed that human umbilica1 cord blood CD34 stern cells separated from human umbilical cord blood mononuclear cells by MACs technology undergo differentiationpotency affecting by the addition of fibroblast and hepatocyte cellextracts to the treated groups groups. The expansion and differentiation potentiality to hepatic cells analyzed by DNAanalysis and compared with controlThe present study showed that stem progenitor cells purification were performed by phenotypic ana ysis in Flow Cytometry using C:D34-FTTC, human kit showed cell purity (76.l2±4.02) % (photo.5). The new selected CD34+ cells were spherical and remains in itgrowth medium were resuspended in 10% and changed every 3 days for 10 to 14 days of incubation at 37°C and 5% CO2 incubator (photo.6) Following treatment with fibroblast cell extract, the