Some Immunochemical studies on microbial exposure of Rabbits

The objective of the present study was to evaluate the changes in theconcentrations of the major blood proteins and serum IL-6 level associated with experimental E. coli and S. aureus infection in rabbits. For that, 18male Dutch rabbits (12 wk old) were divided into: 6 rabbits in the controlgroup (group 1) whereas in one assay group: 6 rabbits wereintraperitoneally injected with abacterial suspension of enteropathogenicE. coli strain type 0157: H (group IT) and the other assay group: 6 rabbitsinjected by the same way but with S. aureus bacterial suspension (groupIll).The collected samples were then centrifuged at 10.000 x g in ordinarycentrifuge for serum separation.Serum IL-6, total protein, albumin and globulin fractionsconcentrations as well as A/G ratio (protein electrophoresis) were measuredbefore (Oh) and after 24, 48, 72 and 96 hours from the bacterial injection.Determination of serum IL-6 concentrations:Tn the present study, the IMMULITE and TMMULITE 1000 Analyzerswas used for the quantitative measurement of interleukin 6 (IL-6) in rabbitserum during a primary EPEe 0157: H and pathogenic Staph. aureusinfections.Although all the infected rabbits show no clinical signs of disease,both S. aureus and E. coli stimulate the production ofIL-6 comparing withthe control group and it began to increase dramatically till clearly observedin the s” and ill day of the infection and there was a significant increase ingroup lTI (exposed to S. aureus) than group IT (exposed to E. coli). I