Molecular Biological and Biochemical Studies on the Effect of Some Natural Compounds on H5NI Avian Fiu Virus Neuraminidase

Influenza viruses are ubiquitous worldwide and they producesignificant annual morbidity and mortality with potentiallydevastating consequences for human and animal health and theglobal economy.Type A viruses are further divided into subtypes according tothe combination of two main envelope glycoproteins thehemagglutinin (HA) and neuraminidase (NA).The role of influenza virus NA may be to facilitate mobilityof the virus both to and from the site of infection, it is responsiblefor catalyzing the cleavages of a (2-6) or (2-3) ketosidic linkage thatexists between a terminal SA and an adjacent sugar residue on thesurface of the cell.It is generally believed that inhibition of NA markedlyprevent the viral release from the host cell. Recent studies showedthat plant extracts possess antiviral potentials against viral strainsresistant to conventional antiviral agentsThe present work was carried out to study the molecular andbiochemical effect of some natural compounds (green tea extractand Licorice extracts) on neuraminidase enzyme of Egyptian avianinfluenza H5Nl in infected tissue culture.During this study AI virus was propagated in ECE thenallantoic fluids were harvested and confirmed by HA assay. Furthermore cytopathic effect of avian influenza virus H5N I on MDCKtissue culture was detected under the microscope at 40X then thevirus had been titrated.