Abstract: |
In this study we prepared E. coli vaccine by twodifferent methods:1. Sonicated E. coli vaccine.2. Formalin inactivated E. coli vaccinewhere the breeder hens were vaccinated at age of 20and 25 weeks. After that were evaluate the antibody titre usingELISA test and IHT (indirect heam agglutination) test.The results reveled thatI. Immune response to vaccination:I-Sonicated E.coli vaccineBreedersELISA titre was 8.86, 9.50, 9.79 and 9.84 at 1 S\ 2nd, 3rd and 4thweeks post vaccination while GMT titre was l.99, 2.35,2.59 and 2.71 respectively.Eggs:,ELISA titer was 8.1, 8.4, 8.7 and 8.86 at 1st, 2nd, 3rd and 4thweeks post vaccination while GMT titre was l.38, l.7, 2.04and 2.43 respectively.Chicks: ELISA titre were 8.81,7.29,6.8 ,6.8 and 5.79 at 1,7, 14, 21 and 28 days old post hatching while GMT titrewere 2.1, l.92, l.62, l.32 and l.20 respectively.
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