Advanced Techniques to Study Some Bacterial Agents Involved in Bovine Respiratory Infection

Respiratory disease considered one of the most causes of economicloss in cattle industry especially due to bacterial and mycoplasmalinfection.A total of 556 samples were collected from cattle from different cattlefarms and slaughter houses at Sharkia, Cairo Govemorate, Egypt, including169 nasal swabs, 129 tracheal samples, 129 lung and 129 bronchial lymphnodes. The highest incidence was from bacteria 37.9%, followed byMycoplasma (17%), Acholeplasma (10.8%), while mixed infection was(21.4%), Mycoplasma species was isolated by percentage of (17%).Diseased cattle revealed the highest incidence than apparently healthy onereaching 22.6%, 9.9% respectively. The highest isolation rate was fromlung of diseased and apparently healthy animals 24.8%, while lymph nodesshowed the lowest isolation rate 10%.Biochemical identification revealed that M arginini was the highestincidence by percentage of 7%, followed by M bovis 6.5%, M bovithinis1.9% while the lowest incidence was from M bovigenitslium 1.6%.Bacteria species were isolated by percentage of 37.9%. The incidenceof bacteria from diseased cattle were 43% while from the apparentlyhealthy one were 30.9%, nasal swabs revealed the highest incidence ofbacteria reaching 45.5%, while bronchial lymph nodes were the lowestincidence reaching 26.3%.