A Biochemical Study On The Genotoxie Effect Of Some Antibiotics In Mice

The genotoxic effect is one of the serious risks results from theuse of pharmaceuticals and result in genetic damage. The inductionof genetic damage is a critical step in the production of differentgenetic diseases in future generations, birth defects and contributesto somatic diseases such as cancer in the present generation.Therefore it is necessary to evaluate the genotoxic potential of drugsto evaluate their ability to cause cancer and other defects.The genotoxic effect of the two antibiotics (ciprofloxacin andrifampicin) administration and their combination with curcumintreatment in male mice to ameliorate the ciprofloxacin andrifampicin -induced genotoxic effects was investigated throughdetermination of the DNA damaging effect,the serum level of 8-hydroxy-2’-deoxyguanosine(8-0HdG),comet assay and the gene expression of certain enzymes( Glutathione s-transferase u2, Glutathione s-transferase mu 2, catalase, Cytochrome P450 2El, 8- oxoguanine DNA glycosylase 1) in liver tissues. In the present study Animal House, Faculty of Veterinary Medicine, Zagazig University.They were maintained on basal ration and tap water andphotoperiod 12L/12D cycle.Mice were kept at a constant environmental and nutritionalcondition throughout the periods of the experiment.