A Biochemical Study On Hemoglobin

Organic nitrates are among the oldest and most commonly used drugs for the treatment of cardiovascular diseases. When nitrates are metabolized to release NO, there is a considerable coproduction of reactive oxygen species as superoxide radical and reactivForty two male wistar rats weighing about 150-180 g were used in the present study. The experimental animals were divided into seven main groups: A) Group (1) control group:Rats were received normal laboratory diet without any treatment. B) Group (2):Rats of this group were supplied with normal laboratory diet and received orally ISMN with a gastric tube at a dose of 1.7 mg/ kg body weight every day for a period of 2 months. C) Group (3) Rats of this group were supplied with normal laboratory diet andg) Group (7) Rats of this group were supplied with normal laboratory diet and received orally ISMN at the same dose of group (6) followed by vitamin C at a dose of 28.5 mg/ kg body weight for the same period 2 months.At the end of the experimental period 2 months, blood samples were collected in clean centrifuge tubes for biochemical investigations of total hemoglobin, methemoglobin, carboxyhemoglobin, saturated oxygen, oxygen content, hematocrit, red blood cells, whiThe obtained results can be summarized as follow:1- Hemoglobin: There was a significant decrease in hemoglobin comparing with control group while a significant increase in groups given vitamin C comparing with ISMN treated groups of the same dose.2- MetheOxygen content showed a significant decrease in all groups compared with that of the control group, while a significant increase in the group given vitamin C+ ISMN 6.8mg/kg comparing with ISMN treated groups of the same dose.6- Hematocrit: All animal grouThere was a significant increase in nitric oxide concentration was observed in all treated rat groups compared with that of the control group, while significant decrease in nitric oxide concentration was observed between groups given vitamin C comparing w