| Abstract: |
Summary:-Hepatitis B is a potentially life-threatening liver infection caused by the hepatitis B virus. It is a major global health problem and the most serious type of viral hepatitis. It can cause chronic liver disease and puts people at high risk of deLamivudine one of the most antiviral drugs has good compliance for HBV patient and used also for HIV patients with highly active antiretroviral therapy (HAART) medication.The present study was carried out to evaluate the most important side effects of lamivudine and its immunological character in male albino rats. These effects were studied through estimation of liver and kidney function parameters, humoral immunity and phThe experimental design was as follows:Fifty adult male albino rats were divided into 2 groups, control and treated groups (25 rats each). Lamivudine was administered to the treated group for 60 days (10 mg/kg b.wt.) once daily.Effect on hematological parameters:The male albino rats were administered lamivudine once daily dose and blood samples were taken from eye canthus in heparinized tubes for estimation of RBCs parameters and total, differential leukocytic count on the 30th, 60th day.We found the incidence of anemia and leukopenia on the 30th day but all parameters returned to its normal levels on the 60th day which reveals no serious side effects of lamivudine.Effect on humoral immunity:The male albino rats were administered lamivudine once daily dose and serum samples were taken from them for estimation of IgG and IgM on the 15th, 30th, 45th, 60th day.Results of this study showed high significant increase in IgG and no significant change in IgM which reveals immuno stimulation effect of lamivudine.Effect on urea and creatinine:The male albino rats were administered lamivudine once daily dose and serum samples were taken from them for estimation of urea and creatinine on the 15th, 30th, 45th, 60th day.It was found that there is non significant changes in urea and significant decrease in creatinine between treated and control group and this reveals the high safety profile of lamivudine on kidney.Effect on liver enzyme:The male albino rats were administered lamivudine once daily dose and serum samples were taken from them for estimation of liver enzymes on the 15th, 30th, 45th, 60th day.Results of this study demonstrated that, there is non significant changes in AST, ALT, ALP between treated and control groups even there was significant decrease in ALT, but within normal value.Effect on total proteins, albumin and globulines:The male albino rats were administered lamivudine once daily dose and serum samples were taken from them for estimation of total proteins and albumin on the 15th, 30th, 45th, 60th day.The present results revealed a high significant increase in total protein and total globulines. Non significant change was observed in total albumin among treated and control group.Effect on phagocytic capacity:The male albino rats were administered lamivudine once daily dose and blood samples were taken from them for estimation of phagocytic percent and phagocytic index on the 30th, 60th day.The present work recorded high significant increase in phagocytic percent and phagocytic index in the treated group compared to those of the control group which revealed high efficacy of lamivudine in restoration and activation of phagocytic function of m
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